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High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)

High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)
File Size:
145.09 kB
Author:
Aneta Spórna-Kucab, S. Ignatova, I. Garrard, S. Wybraniec
Email:
anetasporna[at]chemia[dot]pk[dot]edu[dot]pl
Date:
13 August 2013
Downloads:
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Abstract: A study on a separation of betanin and its decarboxy- and dehydro-derivatives obtained from red beet roots (Beta vulgaris L.) using analytical high-performance countercurrent  chromatography (HPCCC — Dynamic Extractions Ltd., UK) was performed. Th e HPCCC process was accomplished in the ‘tail to head’ mode with three highly polar solvent systems with high salt concentrations: 1-propanol-acetonitrile-saturated ammonium sulphate-water (v/v/v/v, 1:0.5:1.2:1); ethanol-acetonitrile-1- propanol-saturated ammonium sulphate-water (v/v/v/v/v, 0.5:0.5:0.5:1.2:1) and ethanol-1-butanol-acetonitrile-saturated ammonium sulphate-water (volume ratio), 0.5:0.5:0.5:1.2:1). HPLC analysis was performed in a conventional reversed phase mode with diode-array (DAD) detection to characterize the composition of obtained fractions. Th e applied solvent systems enabled the separation of the betalain pigments with high effi ciency for the fi rst time. In the mode of separation selected, the more hydrophobic compounds eluted fi rst as expected. Moreover, for the fi rst time, the applied HPCCC solvent systems generated a separation of 2-decarboxy-betanin from 17- and 2,17-bidecarboxy-betanin as well as from neobetanin and betanin.

Keywords: betanin, betalains, betacyanins, countercurrent chromatography, Beta vulgaris L

Area: Natural Sciences

BibTeX:

@article{Kucab2012,
  author = {Aneta Spórna-Kucab and S. Ignatova and I. Garrard and S. Wybraniec},
  title = {High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)},
  journal = {Challenges of Modern Technology},
  year = {2012},
  volume = {3},
  number = {1},
  pages = {57--61},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/81-high-salt-solvent-systems-in-separation-of-betanin-and-its-derivatives-from-red-beet-beta-vulgaris-l-by-high-performance-countercurrent-chromatography-hpccc}
}
 
 

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