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Application of culture dependent methods and culture-independent methods (DGGE analysis) to study Lactic acid bacteria ecology of Ivorian fermented fi sh Adjuevan

Application of culture dependent methods and culture-independent methods (DGGE analysis) to study Lactic acid bacteria ecology of Ivorian fermented fi sh Adjuevan
File Size:
175.32 kB
Author:
Kouakou Amenan Clementine, Kouadio Florent Nguessan, Dadier A. Thomas, Marcellin Koffi Dje, Didier Montet
Email:
labish76[at]yahoo[dot]fr
Date:
13 August 2013
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Abstract: The occurrence of lactic acid bacteria fl ora (LAB) was investigated on Ivorian fermented fi sh Adjuevan products produced with sea fi sh at diff erent salt concentration (10, 15, 20, 25, 30%) according two traditional methods. LAB biodiversity was investigated using traditional culture-dependent method and culture-independent method (DGGE). LAB isolates were Lactobacillus fermentum 54%, Leuconostoc lactis subsp lactis 27%, Pediococcus pentosaceus 19% according method 1 and Pediococcus pentosaceus 61%, Lactococcus garviae 32%, Streptococcus diffi cilis 7% for method 2. Th e results of culture-independent method using DGGE patterns and sequencing of DNA bands revealed a higher number of lactic acid bacteria species even if the identifi cation of several lactic acid bacteria species were not possible by traditional microbiological procedures. LAB were Lactobacillus delbrueckii subsp bulgaricus, Lactobacillus helveticus, Leuconostoc lactis, Lactococcus raffi nolactis. Microfl ora was infl uence by salt percentage and more by the method of fermentation used. Th e molecular method DGGE which used three primers Lac1, Lac2GC and Lac3 to study LAB biodiversity directly in the fermented fi sh matrix, have allowed us to get a more complete picture of the dominant lactic acid bacteria diversity in these fermented product Adjuevan.

Keywords: Adjuevan; Fermented fi sh; PCR-DGGE, Ivory Coast

Area: Natural Sciences

BibTeX:

@article{Clementine2012,
  author = {Kouakou Amenan Clementine and Kouadio Florent Nguessan and Dadier A. Thomas and Marcellin Koffi Dje and Didier Montet},
  title = {Application of culture dependent methods and culture-independent methods (DGGE analysis) to study Lactic acid bacteria ecology of Ivorian fermented fish Adjuevan},
  journal = {Challenges of Modern Technology},
  year = {2012},
  volume = {3},
  number = {1},
  pages = {51--56},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/80-application-of-culture-dependent-methods-and-culture-independent-methods-dgge-analysis-to-study-lactic-acid-bacteria-ecology-of-ivorian-fermented-fi-sh-adjuevan}
}
 
 

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