Novel designs and technologies for cell engineering

Novel designs and technologies for cell engineering
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Karina Ziółkowska, Michał Chudy, Artur Dybko, Zbigniew Brzózka
13 August 2013
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Abstract: Microfl uidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfl uidic channels for culture medium fl ow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.

Keywords: lab-on-a-chip, multicellular tumor spheroid, cancer, in vitro cell culture, cell engineering

Area: Natural Science


  author = {Karina Ziółkowska and Michał Chudy and Artur Dybko and Zbigniew Brzózka},
  title = {Novel designs and technologies for cell engineering},
  journal = {Challenges of Modern Technology},
  year = {2011},
  volume = {2},
  number = {4},
  pages = {54--60},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/68-novel-designs-and-technologies-for-cell-engineering}

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