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Mechanobiology of soft tissues: FT-Raman spectroscopic studies

Mechanobiology of soft tissues: FT-Raman spectroscopic studies
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205.29 kB
Author:
Marlena Gąsior-Głogowska, Małgorzata Komorowska, Jerzy Hanuza, Mirosław Mączka, Romuald Będziński, Magdalena Kobielarz
Email:
marlena[dot]gasior-glogowska[at]pwr[dot]wroc[dot]pl
Date:
13 August 2013
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Abstract: FT-Raman spectroscopy was used to investigate microstructural changes in the secondary protein structure of soft tissues subjected to increasing levels of macroscopic strain. Main protein bands at 938 cm-1 assigned as ν(C–C), 1668 cm-1 — amide I and 1268 cm-1 — amide III are sensitive to applied strain and undergo wavenumber shifting. Other main vibrational modes at 1004 cm-1 assigned to the phenyl ring breathing mode and 2940 cm-1 (n(CH3,CH2)) remain unaltered. Spectroscopic results were compared with the mechanical relations obtained from the standard protocol of uniaxial tensile tests carried out in a testing machine. A clear correlation between Raman band shifting and the level of mechanical stress was established. Initially the load is transferred through elastin and then gradually also by collagen. It was proved that transferring loads by soft tissues involves changes in structural protein conformation. Th is process was described in detail for a tendon. It was also confi rmed that mechanical properties of soft tissues depend on collagen and elastin fi bers orientation.

Keywords: Raman spectroscopy, collagen, tendon, aorta, soft tissues, mechanical properties, uniaxial tensile test

Area: Biomedical Engineering

BibTeX:

@article{Gasior2011,
  author = {Marlena Gąsior-Głogowska and Małgorzata Komorowska and Jerzy Hanuza and Mirosław Mączka and Romuald Będziński and Magdalena Kobielarz},
  title = {Mechanobiology of soft tissues: FT-Raman spectroscopic studies},
  journal = {Challenges of Modern Technology},
  year = {2011},
  volume = {2},
  number = {3},
  pages = {8--11},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/43-mechanobiology-of-soft-tissues-ft-raman-spectroscopic-studies}
}
 
 

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