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Spiral-coil countercurrent chromatographic separation of betanin and betanidin ethyl-esters monitored by continuous ESI-MS/MS injection and LC-ESI-MS/MS

Spiral-coil countercurrent chromatographic separation of betanin and betanidin ethyl-esters monitored by continuous ESI-MS/MS injection and LC-ESI-MS/MS
File Size:
1.18 MB
Author:
Dorota Tuwalska, Dominika Szot, Sławomir Wybraniec, Peter Winterhalter, Gerold Jerz
Email:
dtuwal[at]chemia[dot]pk[dot]edu[dot]pl
Date:
11 March 2014
Downloads:
19 x

Abstract: Betalains present in Beta vulgaris L. (Chenopodiaceae) have a high potential as natural pigments for food applications. They have been used as a substitute of synthetic dyes in processing of gelatine, strawberry yogurts, ice creams, fruits cocktails, candies and biscuits. The stability of betalains is strongly influenced by numerous factors – sugar content, and impact of light, oxygen, water activity, pH and temperature. A mixture of betacyanin mono-, di- and tri-ethyl-esters was separated by a large-volume spiral-coil countercurrent chromatography (spCCC) prototype which yielded fractions continuously off-line injected to an  ESI-MS/MS device. This method yielded a reconstituted pigment profile and finally enabled the MS-target-guided isolation procedure for the whole spCCC-experiment. The individual fractions were analyzed by LC/ESI-MS/MS. Betanin and betanidin mono-, di- and tri-ethyl-esters as well as other decarboxylated derivatives were detected in the chromatograms. The ethyl-esters of betanin were only partially fractionated, but clearly fractionated from the ethyl-esters of betanidin which will be of great value for biological evaluations.

Keywords: spiral-coil countercurrent chromatography (spCCC), betacyanin ethyl-esters, betalains, betacyanins, betanin, betanidin

Area: Chemistry and Pharmacy

BibTeX:

@article{Tuwalska2013,
  author = {Dorota Tuwalska and Dominika Szot nad Sławomir Wybraniec and Peter Winterhalter and Gerold Jerz},
  title = {Spiral-coil countercurrent chromatographic separation of betanin and betanidin ethyl-esters monitored by continuous ESI-MS/MS injection and LC-ESI-MS/MS},
  journal = {Challenges of Modern Technology},
  year = {2013},
  volume = {4},
  number = {4},
  pages = {21--25},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/165-spiral-coil-countercurrent-chromatographic-separation-of-betanin-and-betanidin-ethyl-esters-monitored-by-continuous-esi-ms-ms-injection-and-lc-esi-ms-ms}
}
 
 

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