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Liquid chromatographic techniques in separation of betacyanins and their derivatives from red beet roots (Beta vulgaris L.)

Liquid chromatographic techniques in separation of betacyanins and their derivatives from red beet roots (Beta vulgaris L.)
File Size:
227.93 kB
Author:
Aneta Spórna, P. Stalica, G. Jerz, M. Szaleniec, S. Wybraniec
Email:
swybran[at]chemia[dot]pk[dot]edu[dot]pl
Date:
15 August 2013
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Abstract: A study on separation of betacyanins and their decarboxylated as well as dehydrogenated derivatives obtained from red beet roots (Beta vulgaris L.) in high pressure liquid chromatography (HPLC) and ion-pair high-speed counter-current chromatography (IP-HSCCC) was performed. Th e IP-HSCCC process was accomplished in the ‘head-to-tail’ mode in a solvent system composed of butanol — acetonitrile — water (5:1:6 v/v/v, acidifi ed with 0.7% trifl uoroacetic acid). Th e HPLC separation was performed in a typical reversed phase mode with mass spectrometry (ESI-MS) and diode-array (DAD) detection. Th e chromatographic profi les of betalains obtained in these two techniques were signifi cantly diff erent. In HPLC, the most polar compounds, like betanin and isobetanin, eluted before less polar decarboxylated and dehydrogenated derivatives (mostly degradation products of betanin). In IP-HSCCC, the dehydrogenated derivatives were eluted faster then their non-dehydrogenated analogues. It was observed for the fi rst time that betanin and neobetanin (14,15-dehydrogenated betanin), which are present in many plants containing betalains, had reversed elution orders during chromatographic separation by these two techniques.

Keywords: betanin, betalains, betacyanins, ion-pair counter-current chromatography, perfl uorinated carboxylic acids

Area: Natural Sciences

BibTeX:

@article{Sporna2010,
  author = {Aneta Spórna and P. Stalica and G. Jerz and M. Szaleniec and S. Wybraniec},
  title = {Liquid chromatographic techniques in separation of betacyanins and their derivatives from red beet roots (Beta vulgaris L.)},
  journal = {Challenges of Modern Technology},
  year = {2010},
  volume = {1},
  number = {1},
  pages = {19-22},
  url = {http://www.journal.young-scientists.eu/index.php/isuues/file/122-liquid-chromatographic-techniques-in-separation-of-betacyanins-and-their-derivatives-from-red-beet-roots-beta-vulgaris-l}
}
 
 

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